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nebnext companion module for oxford nanopore technologies ligation sequencing  (New England Biolabs)


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    Structured Review

    New England Biolabs nebnext companion module for oxford nanopore technologies ligation sequencing
    Nebnext Companion Module For Oxford Nanopore Technologies Ligation Sequencing, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+companion+module/NEBNext+Companion+Module+for+Oxford/pmc13125923-18-0-0
    Average 97 stars, based on 32 article reviews
    nebnext companion module for oxford nanopore technologies ligation sequencing - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Sequencing:

    Article Title: A Bioinformatic Pipeline for Consensus Taxonomic Classification of Long-Read Amplicons
    Article Snippet: PCR products were pooled into libraries at roughly equal concentrations, as estimated from gel band intensities, and purified using a Monarch PCR & DNA cleanup kit (NEB). .. ONT sequencing was performed at SeqCenter (Pittsburg, PA), with library preparation using the PCR-free ONT Ligation Sequencing Kit (SQK-NBD114.24) with NEBNext® Companion Module (E7180L), sequencing using R10.4.1 flow cells in 400-bps sequencing mode with minimum read length of 200 bp and no adaptive sampling, and super-accurate base calling using Guppy v6.5.7 (model dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg) with no quality trimming. ..

    Article Title: Cranberry juice potentiates sensitivity of uropathogenic Escherichia coli (UPEC) strains to fosfomycin and decreases occurrence of spontaneous resistance.
    Article Snippet: .. For long-read sequencing, libraries were prepared without PCR amplification using the Oxford Nanopore Technologies (ONT) Native Barcoding Kit (SQK-NBD114.24) in combination with the NEBNext Companion Module (E7180L), following the manufactur er’s protocols. ..

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, Ipswich, MA, USA), NEBNext Quick Ligation Reaction Buffer, and Blunt/TA Ligase Master Mix (New England Biolabs). .. Sequencing was performed on a MinION Mk1B platform (Oxford Nanopore Technologies Ltd) using an R10 flow cell.

    Article Title: Whole-Genome Long-Read Sequencing for Rapid Comprehensive Molecular Diagnostics of Brain Tumors
    Article Snippet: Genomic DNA was extracted from fresh or frozen biopsies using the DNeasy Blood & Tissue kit (Qiagen). .. Sequencing libraries were prepared with the NEBNext companion module (New England Biolabs) and ligation sequencing kit (SQK-LSK114, ONT). ..

    Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology
    Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180S; New England Biolabs), following the manufacturers’ protocols. ..

    Article Title: Unraveling the potential of short and long read sequencing for human genome profiling
    Article Snippet: .. We prepared the ONT library from 1.0 μg gDNA input (with no short read eliminator treatment), sheared using Megaruptor 3 (Diagenode) at speed 33 in a volume of 100 μL, using Ligation Sequencing kit V14 (ONT) and NEBNext Companion Module for ONT Ligation Sequencing (NEB), with clean-up steps using AMPure XP Beads (included with the ONT Ligation kit), all according to the manufacturers’ instructions. .. We performed QC using Qubit dsDNA HS assay kit (Thermo Fisher).

    Article Title: Genome Dynamics and Chromosome Structural Variations in Histoplasma ohiense, a fungal pathogen of humans.
    Article Snippet: .. 116 117 Nanopore sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT) Ligation 118 Sequencing Kit (SQK-NBD114.24) with the NEBNext® Companion Module (E7180L) to manufacturer’s speci-119 fications. ..

    Polymerase Chain Reaction:

    Article Title: A Bioinformatic Pipeline for Consensus Taxonomic Classification of Long-Read Amplicons
    Article Snippet: PCR products were pooled into libraries at roughly equal concentrations, as estimated from gel band intensities, and purified using a Monarch PCR & DNA cleanup kit (NEB). .. ONT sequencing was performed at SeqCenter (Pittsburg, PA), with library preparation using the PCR-free ONT Ligation Sequencing Kit (SQK-NBD114.24) with NEBNext® Companion Module (E7180L), sequencing using R10.4.1 flow cells in 400-bps sequencing mode with minimum read length of 200 bp and no adaptive sampling, and super-accurate base calling using Guppy v6.5.7 (model dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg) with no quality trimming. ..

    Article Title: Cranberry juice potentiates sensitivity of uropathogenic Escherichia coli (UPEC) strains to fosfomycin and decreases occurrence of spontaneous resistance.
    Article Snippet: .. For long-read sequencing, libraries were prepared without PCR amplification using the Oxford Nanopore Technologies (ONT) Native Barcoding Kit (SQK-NBD114.24) in combination with the NEBNext Companion Module (E7180L), following the manufactur er’s protocols. ..

    Article Title: Genome Dynamics and Chromosome Structural Variations in Histoplasma ohiense, a fungal pathogen of humans.
    Article Snippet: .. 116 117 Nanopore sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT) Ligation 118 Sequencing Kit (SQK-NBD114.24) with the NEBNext® Companion Module (E7180L) to manufacturer’s speci-119 fications. ..

    Ligation:

    Article Title: A Bioinformatic Pipeline for Consensus Taxonomic Classification of Long-Read Amplicons
    Article Snippet: PCR products were pooled into libraries at roughly equal concentrations, as estimated from gel band intensities, and purified using a Monarch PCR & DNA cleanup kit (NEB). .. ONT sequencing was performed at SeqCenter (Pittsburg, PA), with library preparation using the PCR-free ONT Ligation Sequencing Kit (SQK-NBD114.24) with NEBNext® Companion Module (E7180L), sequencing using R10.4.1 flow cells in 400-bps sequencing mode with minimum read length of 200 bp and no adaptive sampling, and super-accurate base calling using Guppy v6.5.7 (model dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg) with no quality trimming. ..

    Article Title: Psychrobacillus syltrankelensis sp. nov., a new species isolated from soil in the North Caucasus, Russia.
    Article Snippet: Genomic DNA for Oxford Nanopore sequencing was isolated using the MagBeads FastDNA Kit for Microbiome (MP Biomedicals, Santa Ana, CA, USA) according to the manufacturer’s instructions. .. Libraries were prepared following the manufacturer’s protocol with the Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies Ltd, Oxford, UK), the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, Ipswich, MA, USA), NEBNext Quick Ligation Reaction Buffer, and Blunt/TA Ligase Master Mix (New England Biolabs). .. Sequencing was performed on a MinION Mk1B platform (Oxford Nanopore Technologies Ltd) using an R10 flow cell.

    Article Title: Whole-Genome Long-Read Sequencing for Rapid Comprehensive Molecular Diagnostics of Brain Tumors
    Article Snippet: Genomic DNA was extracted from fresh or frozen biopsies using the DNeasy Blood & Tissue kit (Qiagen). .. Sequencing libraries were prepared with the NEBNext companion module (New England Biolabs) and ligation sequencing kit (SQK-LSK114, ONT). ..

    Article Title: Long-Read Haplotype Phasing Resolves Allelic Configuration as a Missing Layer of Precision Oncology
    Article Snippet: Samples with a high proportion of short DNA fragments were further processed using the BluePippin High-Pass DNA Size Selection System (Sage Science) to enrich for fragments greater than 5 kb, suitable for nanopore sequencing. .. Sequencing libraries were prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies) in combination with the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (E7180S; New England Biolabs), following the manufacturers’ protocols. ..

    Article Title: Unraveling the potential of short and long read sequencing for human genome profiling
    Article Snippet: .. We prepared the ONT library from 1.0 μg gDNA input (with no short read eliminator treatment), sheared using Megaruptor 3 (Diagenode) at speed 33 in a volume of 100 μL, using Ligation Sequencing kit V14 (ONT) and NEBNext Companion Module for ONT Ligation Sequencing (NEB), with clean-up steps using AMPure XP Beads (included with the ONT Ligation kit), all according to the manufacturers’ instructions. .. We performed QC using Qubit dsDNA HS assay kit (Thermo Fisher).

    Article Title: Genome Dynamics and Chromosome Structural Variations in Histoplasma ohiense, a fungal pathogen of humans.
    Article Snippet: .. 116 117 Nanopore sample libraries were prepared using the PCR-free Oxford Nanopore Technologies (ONT) Ligation 118 Sequencing Kit (SQK-NBD114.24) with the NEBNext® Companion Module (E7180L) to manufacturer’s speci-119 fications. ..

    Sampling:

    Article Title: A Bioinformatic Pipeline for Consensus Taxonomic Classification of Long-Read Amplicons
    Article Snippet: PCR products were pooled into libraries at roughly equal concentrations, as estimated from gel band intensities, and purified using a Monarch PCR & DNA cleanup kit (NEB). .. ONT sequencing was performed at SeqCenter (Pittsburg, PA), with library preparation using the PCR-free ONT Ligation Sequencing Kit (SQK-NBD114.24) with NEBNext® Companion Module (E7180L), sequencing using R10.4.1 flow cells in 400-bps sequencing mode with minimum read length of 200 bp and no adaptive sampling, and super-accurate base calling using Guppy v6.5.7 (model dna_r10.4.1_e8.2_400bps_modbases_5mc_cg_sup.cfg) with no quality trimming. ..

    Amplification:

    Article Title: Cranberry juice potentiates sensitivity of uropathogenic Escherichia coli (UPEC) strains to fosfomycin and decreases occurrence of spontaneous resistance.
    Article Snippet: .. For long-read sequencing, libraries were prepared without PCR amplification using the Oxford Nanopore Technologies (ONT) Native Barcoding Kit (SQK-NBD114.24) in combination with the NEBNext Companion Module (E7180L), following the manufactur er’s protocols. ..



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